伊红美蓝培养基鉴别大肠杆菌和志贺菌时一区为什么黄色
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伊红美蓝培养基鉴别大肠杆菌和志贺菌时一区为什么黄色
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It's principle is lactose fermentation...Lactose fermenters metabolise the lactose in the media and produce acid byproducts,causing a color change in the colony.Strong acid production by organisms such as E.coli results in a metallic green sheen.Weaker fermentation of lactose results in colonies with a pinkish-purple color.Colonies of nonlactose fermenters remain colorless,or at least are no darker than the color of the media.Therefore,it shouldn't look yellow:-(
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